Plant Regeneration from Callus and Adventitious Root Segments of Pulsatilla Koreana Nakai
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چکیده
منابع مشابه
Pulsatilla saponin D: the antitumor principle from Pulsatilla koreana.
By bioassay-guided separation, an already known saponin, Pulsatilla saponin D was isolated from the root of Pulsatilla koreana Nakai as a antitumor component when evaluated by in vivo antitumor activity as well as in vitro cytotoxic activity test. It showed potent inhibition rate of tumor growth (IR, 82%) at the dose of 6.4 mg/kg on the BDF1 mice bearing LLC cells.
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Pulsatilla koreana, a species endemic to Korea, is an important herb used in traditional medicine to treat amoebic dysentery and malaria. In the present study, 23 oleanane-type triterpenoid saponins 1-23 and eight lupane-type triterpenoid saponins 24-31 were isolated from the roots of P. koreana. Their structures were elucidated on the basis of spectroscopic data. The methanol extract and isola...
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The capacity of plants to regenerate is impressive. Plant cells can undergo somatic embryogenesis in culture, forming an embryo from a single cell or a group of somatic cells. In addition, plants exhibit de novo organogenesis, in which new organs and even entire plants are produced from other organs upon wounding. Almost as remarkable as the phenomenon of plant regeneration is how little we kno...
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Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...
متن کاملBiochemical Basis of Adventitious Root Formation on Etiolated Stem Segments
Segments (2.5-cm-long) of Populus nigra L. obtained from etiolated axillary branches did not root in water or auxin alone, but rooted in 0.5% ribose, glucose and sucrose and more profusely with 0.1 mg/1 in dole-acetic acid (IAA) or in dolebutyric acid (IBA) added to the medium. 5-fluorodeoxyuridine (FUDR), 5-fluorouracil (FU), actinomycin-D and cycloheximide inhibited rooting. The deoxyribonucl...
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ژورنال
عنوان ژورنال: Journal of Plant Biotechnology
سال: 2007
ISSN: 1229-2818
DOI: 10.5010/jpb.2007.34.2.153